Fig. 2: Fungal-specific CD4 T cells are recruited to the brain late post-infection.
a Schematic of adoptive transfer model used in this study. b Frequency of transferred fungal-specific CnT.II CD4 T cells in the brain (n = 6 mice day 0, n = 9 mice day 7, **P = 0.0021), spleen (n = 7 mice day 0, n = 9 mice day 7, **P = 0.0021), lung (n = 7 mice day 0, n = 9 mice day 7, *P = 0.0212) and cLN (n = 6 mice day 0, n = 9 mice day 7, **P = 0.0052), c the frequency of divided (CFSElow) CnT.II cells in the brain (n = 6 mice day 7), spleen (n = 5 mice day 0, n = 6 mice day 7, **P = 0.0027), lung (n = 5 mice day 0, n = 6 mice day 7, ****P < 0.0001) and cLN (n = 6 mice day 0, n = 6 mice day 7, **P = 0.0024) and (d) frequency of CD44 expression within CnT.II cells in the brain (n = 6 mice day 7), spleen (n = 5 mice day 0, n = 6 mice day 7, ****P = 0.0001), lung (n = 5 mice day 0, n = 6 mice day 7, ****P = 0.0001) and cLN (n = 3 mice day 0, n = 6 mice day 7, ***P = 0.0002). Data is pooled from two independent experiments and analysed by two-way ANOVA. e Number of brain-infiltrating CnT.II CD4 T cells relative to brain fungal burden in mice infected with low (open symbol), medium (grey symbol) and high (closed symbol) dose of C. neoformans. Each point represents an individual animal. Data analysed by simple linear regression. f Total number of CD4 T cells and fungal-specific CnT.II T cells in the meninges of uninfected (n = 3 mice; open symbol) and infected (n = 10 mice; day 7 post-infection, closed symbol) mice, Data pooled from three independent experiments (one uninfected mouse per experiment) and analysed by unpaired t-test. *P = 0.0127.
