Fig. 4: Elimination of SMC shortens the ParB-HT complex recovery time after photobleaching.
From: SMC modulates ParB engagement in segregation complexes in streptomyces
A Representative image showing the photobleaching of the ParB-HT complex stained with Janelia Fluor-549 in young vegetative cells of the wild type control and Δsmc strains (ΔparAB pnatparAB-HT, KP006 and ΔsmcΔparAB pnatparAB-HT KP007, respectively). ParB-HT fluorescence (red) with hyphal cell contour overlaid (for separate chanells see Supplementary Fig. 9A, Supplementary Movie 6 and 7). B Fluorescence recovery analysis. Intensity of fluorescence plotted against the time of analyses. The dotted lines show mean Tau values for each strain. C Tau – recovery half time - the time required for recovery of half fluorescence intensity calculated for the wild type control and Δsmc strains (ΔparAB pnatparAB-HT, KP006 and ΔsmcΔparAB pnatparAB-HT KP007, respectively). Data shown in B and C were collected in 3 independent experiments for 30 ParB-HT complexes of each strain. Statistical analysis was conducted using a two-sided Wilcoxon test, p-value: 0.036. Boxplots show the median value with 1st and 3rd quartiles as box boundaries and whiskers extending to mean +/− 1.5 * IQR. Source data are provided as a Source Data file.
