Fig. 5: ChIP-seq analyses showing diminished ParB-parS binding and spreading in the absence of SMC. | Nature Communications

Fig. 5: ChIP-seq analyses showing diminished ParB-parS binding and spreading in the absence of SMC.

From: SMC modulates ParB engagement in segregation complexes in streptomyces

Fig. 5

ChIP seq was performed using antigen-purified polyclonal ParB antibody and wild type, ΔparB (MD020) and Δsmc strain (TM010). A ChIP-seq detected binding plotted against the chromosomal region of S. venezuelae chromosome containing 17 parS sites in wild type (red), Δsmc (blue) and ΔparB (grey) strain. Insets show selected parS sites. B The average ChIP-seq signal at parS sites and neighbouring regions in strain wild type (red), Δsmc (blue) and ΔparB (grey). C Heatmap of 17 parS sites bound by ParB ordered by the number of reads detected at parS site in the wild type strain. D Average number of 100 bp long regions with enriched ParB binding in strains: wild type and Δsmc. Regions were counted using a 10000 bp rolling window. Source data are provided as a Source Data file.

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