Fig. 6: SMC complex reduces ParB CTPase activity.
From: SMC modulates ParB engagement in segregation complexes in streptomyces
Bar plot mean +/− SD showing the results of CTP hydrolysis assay (phosphate detection) for ParB (2 μM), ParB (2 μM) in the presence of pBSK plasmid with single parS (15 μM), or ParB (2 μM) in the presence of pBSK plasmid with mutated parS (parSmut) (15 μM) in the absence or the presence of FLAG-SMC (0.02 μM), as compared to ParBSMC- (2 μM) variant in the presence of pBSK plasmid with single parS, or in the presence of pBSK plasmid with mutated parS (parSmut), in the absence or in the presence of FLAG-SMC (0.02 μM) (as indicated). Data were obtained from at least 4 independent experimental repeats. A two-sided Student’s t test with Holm correction for multiple testing was used in statistical analysis. p-values: ParB_parS – ParB: 2e-16, ParB_parS – ParB_parS_SMC: 2e-16, ParB_parS_SMC – ParB_SMC: 2.2e-16, ParBSMC-_parS – ParBSMC-_parS_SMC: 0.0097, ParB_parS_SMC - ParBSMC-_parS_SMC: 1, ParB_parS - ParBSMC-_parS: 9.0e-10, ParB_parS_SMC - ParBSMC-_parS: 2.6e-02. Source data are provided in a Source Data file.
