Fig. 4: Lineage 3 S. sonnei induces a stronger neutrophil response in zebrafish. | Nature Communications

Fig. 4: Lineage 3 S. sonnei induces a stronger neutrophil response in zebrafish.

From: Enhanced virulence and stress tolerance are signatures of epidemiologically successful Shigella sonnei

Fig. 4

Total number of macrophages [Tg(mpeg1::Gal4-FF)gl25/Tg(UAS:LIFEACT-GFP)mu271] (a) and neutrophils [Tg(mpx::eGFP)i114] (b) in S. sonnei infected zebrafish larvae, N = 3 biological replicates with >4 larvae per timepoint, per replicate ( >12 in total) (mean ± SEM) Scale bar = 100 µM. Number of neutrophils (c) and macrophages (d) recruited to the hindbrain ventricle (HBV) over time, N = 3 biological replicates with >4 larvae per timepoint, per replicate ( > 12 in total)(mean ± SEM) Scale bar = 200 µM. P values shown in blue highlight lineage-dependent differences. e–g Relative mRNA expression of pro-inflammatory cytokines from infected larvae at 6 h post-infection (hpi), N = 4, performed in technical duplicates (mean ± SEM). Statistics: Significance was tested with a two-way ANOVA with Tukey’s correction ((a–d), only significance where P < 0.05 are displayed due to space limitations), one-way ANOVA with Tukey’s correction (e–g). Source data are provided as a Source Data file.

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