Fig. 1: The global lactylation and histone lactylation increased in FLSs and synovial tissues from RA patients. | Nature Communications

Fig. 1: The global lactylation and histone lactylation increased in FLSs and synovial tissues from RA patients.

From: Histone lactylation promotes rheumatoid arthritis progression by increasing NFATc2 expression and the production of anti-lactylated histone autoantibodies

Fig. 1

A, B The global lactylation levels of FLSs from HC (n = 4), GA patients (n = 5) and RA patients (n = 5). C Immunofluorescence images of Pan Kla (red, bind lactylated proteins), β-Tubulin (green, bind β-Tubulin) and DAPI (blue, bind nucleus) in HC-FLS, GA-FLS and RA-FLS, scale bar = 100 μm. D The levels of global lactylation in RA-FLS treated with lactate (10 mM), sodium lactate (10 mM) or hydrochloric acid for 24 h. E Immunofluorescence images of Pan Kla (red, bind lactylated proteins), β-Tubulin (green, bind β-Tubulin) and DAPI (blue, bind nucleus) of RA-FLS treated with lactate (10 mM) for 24 h, scale bar = 100 μm. F The histone lactylation of FLSs from HC (n = 4), GA patients (n = 5) and RA patients (n = 5). G Immunofluorescence images of H3K9la (red, bind H3K9la), β-Tubulin (green, bind β-Tubulin) and DAPI (blue, bind nucleus) in HC-FLS, GA-FLS and RA-FLS, scale bar = 100 μm. H IHC staining images of H3K9la in synovial tissue from HC, GA patients and RA patients, scale bar = 100 μm. I The H3K9la levels were detected in synovial tissues from HC, GA patients and RA patients by Western blot. J The level of H3K9la in RA-FLS treated with lactate (10 mM) was detected by Western blot. All of the experiments were performed three independent experiments. Data were presented with means ± SD. F Two-way ANOVA with Turkey’s post hoc test, test was two-sided. Source data are provided with this paper.

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