Fig. 4: Inhibition of endogenous and exogenous lactate reduces H3K9la levels and cell migration of RA-FLS.

A Schematic diagram of endogenous lactate inhibition target. B The H3K9la levels of RA-FLS treated with FX-11(10 μM) or transfected with siLDHA were detected by WB. C ChIP-qPCR assay of H3K9la occupancy rates in the NFATc2 promoter region in RA-FLS treated with FX-11(10 μM) or transfected with siLDHA. D RT-qPCR assay of NFATc2 expression in RA-FLS treated with FX-11(10 μM) or transfected with siLDHA. E Scratch migration assay of RA-FLS treated with FX-11(10 μM) or transfected with siLDHA. F Transwell assay of RA-FLS treated with FX-11(10 μM) or transfected with siLDHA. G Schematic diagram of exogenous lactate inhibition target. H The expression of MCT1 in RA-FLS were detected by WB. I The expression of GPR81 in RA-FLS were detected by nucleic acid electrophoresis (upper panel) and WB (lower panel). J The H3K9la levels of RA-FLS treated with sodium lactate (10 mM) or sodium lactate (10 mM) + AZD-3965 (100 nM). K ChIP-qPCR assay of H3K9la occupancy rates in the NFATc2 promoter region in RA-FLS treated with sodium lactate (10 mM) or sodium lactate (10 mM) + AZD-3965 (100 nM). L RT-qPCR assay of NFATc2 expression in RA-FLS treated with sodium lactate (10 mM) or sodium lactate (10 mM) + AZD-3965 (100 nM). M Transwell assay of RA-FLS treated with sodium lactate (10 mM) or sodium lactate (10 mM) + AZD-3965 (100 nM). N Scratch migration assay of RA-FLS treated with sodium lactate (10 mM) or sodium lactate (10 mM) + AZD-3965 (100 nM). B, H–J The experiments were performed three independent experiments. C–F, K–N Data represent three independent experiments. Data were presented with means ± SD. C–F, K–N one-way ANOVA with Turkey’s post hoc test. Source data are provided with this paper.