Fig. 6: Detected the levels of anti-lactylated histone autoAbs in serum of HC, PsA patients and RA patients.

A The levels of lactylated histone antigen in serum were detected by WB. B–E Anti-lactylated histone and anti-histone autoAbs were measured in serum of HC (n = 50), PsA patients (n = 20) and RA patients (n = 50) by ELISA, including total histone (H2B, H3 and H4 together), H2B, H3 or H4. F Anti-H3K9la and anti-H3K9 autoAbs were measured in serum of HC (n = 50), PsA patients (n = 20) and RA patients (n = 50) by ELISA. G–J Correlation between the level of anti-histone autoAbs in serum and Disease Activity Score 28-joint count were evaluated by Pearson correlation coefficient. K Correlation between the level of anti-H3K9la autoAbs in serum and Disease Activity Score 28-joint count (r = 0.5431, p < 0.0001) were evaluated by Pearson correlation coefficient. L The level of anti-H3K9la autoAbs in serum of RA patients in remission (n = 10), RA patients with low activity (n = 5), RA patients with moderate activity (n = 28) and RA patients with high activity (n = 7). M The level of anti-H3K9la autoAb in individual RA patients with different disease course (n = 3). N The level of anti-H3K9la autoAbs in serum of seronegative RA patients (n = 11) and seropositive RA patients (n = 39). O Correlation between Anti-CCP and the level of anti-H3K9la autoAbs in serum were evaluated by Pearson correlation coefficient. A The experiments were performed three independent experiments. Data were presented with means ± SD. B–F two-way ANOVA with Turkey’s post hoc test, test was two-sided. G–K, O tests were two-sided. L One-way ANOVA with Turkey’s post hoc test. N Two-sided unpaired t test. Source data are provided with this paper.