Table 2 The measured binding affinities between ATG16L1 FIR and six mammalian ATG8 family proteins or their mutants by ITC-based binding assays
From: Molecular bases of the interactions of ATG16L1 with FIP200 and ATG8 family proteins
ATG16L1 | ATG8 protein | Kd (μM) |
|---|---|---|
FIR | LC3A | 7.28 ± 0.45 |
FIR | LC3B | 18.10 ± 1.61 |
FIR | LC3C | 6.27 ± 0.58 |
FIR | GABARAP | 8.11 ± 0.48 |
FIR | GABARAPL1 | 4.59 ± 0.44 |
FIR | GABARAPL2 | 179.00 ± 41.50 |
FIR | GABARAPL1(I21Q) | 11.40 ± 1.10 |
FIR | GABARAPL1(K48E) | N.D. |
FIR | GABARAPL1(L50Q) | 41.60 ± 9.01 |
FIR | GABARAPL1(R67E) | N.D. |
FIR(D239R) | GABARAPL1 | 76.00 ± 75.30 |
FIR(I240Q) | GABARAPL1 | 85.20 ± 13.20 |
FIR(E241R) | GABARAPL1 | 56.30 ± 25.90 |
FIR(V242E) | GABARAPL1 | 10.50 ± 0.85 |
FIR(I243Q) | GABARAPL1 | 164.00 ± 140.00 |
