Fig. 1: β-Catenin promotes the expression of ALKBH8.
From: ALKBH8-mediated codon-specific translation promotes colorectal tumorigenesis
a Venn diagram demonstrating the overlap of down-regulated genes in β-catenin knockdown cells and β-catenin-binding targets. b ChIP-qPCR analysis of β-catenin binding to ALKBH8 gene promoter region, with β-actin (ACTB) gene serving as the control. ChIP analysis was performed with antibodies against β-catenin or control IgG and analyzed by qPCR. Mean ± SEM, 3 biological replicates, two-sided t-test. c, d The mRNA level (c) and protein level (d) of ALKBH8 in HCT116 cells with or without β-catenin knockdown. Mean ± SEM, 3 biological replicates, two-sided t-test. The samples derive from the same experiment but different gels for ALKBH8, c-myc, and GAPDH, and another for β-catenin were processed in parallel. e, f The mRNA level (e) and protein level (f) of ALKBH8 in RKO cells with or without β-catenin overexpression. Mean ± SEM, 3 biological replicates, two-sided t-test. The samples derive from the same experiment, but different gels for β-catenin and c-myc, another for ALKBH8 and GAPDH, were processed in parallel. g Schematic of luciferase reporters with wild-type ALKBH8 promoter or TCF4 binding site mutated construct. h Dual-Luciferase assay using constructs with either the wild-type ALKBH8 promoter or a mutated TCF4 binding site in HCT116 cells with or without β-catenin overexpression. Mean ± SEM, 3 biological replicates, two-sided t-test. i ALKBH8 protein expression in intestinal tissue from Apcmin/+ mice. N, normal tissue. T, tumor tissue. j ALKBH8 protein expression in intestinal tissue from AOM/DSS-treated mice. N, normal tissue. T, tumor tissue. The samples derive from the same experiment but different gels for ALKBH8, another for Actin were processed in parallel. k The mRNA level of ALKBH8 in colorectal cancer tissues and adjacent normal tissues from TCGA database (normal = 42, CRC = 286). Mean ± SD, two-sided t-test. l Two-sided Pearson’s correlation analysis of ALKBH8 mRNA and CTNNB1 mRNA in colorectal cancer tissues from TCGA database, 268 samples. Source data are provided as a Source Data file.
