Fig. 3: ALKBH8 promotes intestinal tumorigenesis.
From: ALKBH8-mediated codon-specific translation promotes colorectal tumorigenesis
a H&E staining of small intestines from 24-week-old Apc min/+;Alkbh8 CTL and Apc min/+;Alkbh8 cKO mice. The black arrows indicate the tumors. Statistical analysis of tumor number is shown on the right panel. Mean ± SEM, 6 mice for each group, two-sided t-test. b Workflow of the AOM/DSS-induced cancer model. c Colons from Alkbh8CTL and Alkbh8cKO mice on day 84 of AOM/DSS induction. d Colon tumor number from (c). Mean ± SEM, 6 mice for each group, two-sided t-test. e H&E staining of colons from Alkbh8CTL and Alkbh8cKO mice after AOM/DSS induction. f Immunoblotting analysis of ALKBH8 (A8) expression in CTL and A8-KO HCT116 cells. g The proliferation assay of CTL and A8-KO HCT116 cells. Cell number at day 4 was statistically analyzed. Mean ± SEM, 3 biological replicates, one-way ANOVA with Dunnett’s multiple comparisons test. h Colony formation assay of CTL and A8-KO HCT116 cells. i The statistical analysis of colony numbers in (h). Mean ± SEM, 3 biological replicates, one-way ANOVA with Dunnett’s multiple comparisons test. j Apoptosis analysis of CTL, A8-KO HCT116 cells. Mean ± SEM, 3 biological replicates, one-way ANOVA with Dunnett’s multiple comparisons test. k Immunoblotting analysis of ALKBH8 expression in CTL and A8-OE HCT116 cells. l The proliferation assay of CTL and A8-OE HCT116 cells. Cell number at day 4 was statistically analyzed. Mean ± SEM, 3 biological replicates, two-sided t-test. m Colony formation assay of CTL and A8-OE HCT116 cells. n The statistical analysis of colony numbers in (m). Mean ± SEM, 3 biological replicates, two-sided t-test. o The volume of xenograft tumors from mice implanted with CTL, A8-KO, or A8-OE cells. Mean ± SEM, 6 mice for each group, one-way ANOVA with Dunnett’s multiple comparisons test. p The images of xenograft tumors from mice implanted with CTL, A8-KO, or A8-OE cells. q The statistical analysis of tumor weight in (p). Mean ± SEM, 6 mice for each group, one-way ANOVA with Dunnett’s multiple comparisons test. Source data are provided as a Source Data file.
