Fig. 6: The methyltransferase domain is required for ALKBH8-regulated translation.
From: ALKBH8-mediated codon-specific translation promotes colorectal tumorigenesis
a Schematic of ALKBH8 domain structure and its key amino acids. b Mass spectrometry analysis of cm5U, mcm5U, and mcm5s2U levels in tRNAs from CTL and A8-KO HCT116 cells, 3 biological replicates. c OP-Puro labeling analysis of global translation levels, 3 biological replicates. d Immunoblot analysis of CTL cells, A8-KO cells reconstituted with wild-type A8 or MT-mutant. e Proliferation analysis of CTL cells, A8-KO cells reconstituted with wild-type A8 or MT-mutant. Mean ± SEM, 3 biological replicates, one-way ANOVA with Tukey’s multiple comparisons test. f Colony formation assay of CTL cells, A8-KO cells reconstituted with wild-type A8 or MT-mutant. Mean ± SEM, 3 biological replicates, one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.
