Fig. 1: Impaired oxidative stress response of primary human trophoblasts (hTCs) from preeclamptic (PE) placentas.
From: LAT1-NRF2 axis controls sFlt-1/PlGF imbalance and oxidative stress in preeclampsia
A sFlt-1/PlGF ratio in cell culture medium from hTCs from healthy and PE placentas. B Protein levels of phosphorylated Flt-1, and phosphorylated and total Akt in hTCs derived from healthy or PE placentas. C ATF4 (ATF4) mRNA levels in hTCs from healthy and PE placentas. D Leucine and methionine uptake in hTCs from healthy and PE placentas. E LAT1 protein levels in hTCs from healthy and PE placentas. F Cellular Hg levels in hTCs from healthy and PE placentas after 5 h treatment with 0.9 µM methyl mercury (MeHg). G Viability of hTCs isolated from healthy and PE placentas after treatment with 0.9 µM MeHg. H Gene expression of SLC7A5, NFE2L2, and ABCC1 of hTCs from healthy and PE placentas after 5 h exposure to 0.9 µM MeHg. I Correlation of SLC7A5 (LAT1) and NFE2L2 (NRF2) gene expression in whole placenta lysates. Data represent mean value ± SD. A, B Healthy (N = 5), PE (N = 5); C Healthy (N = 14), PE= (N = 11); D Healthy (N = 6), PE (N = 5); E Healthy (N = 5), PE (N = 5); F Healthy (N = 21), PE (N = 13); G Healthy (N = 20), PE (N = 13), H (Healthy w/o MeHg (N = 12), Healthy with MeHg (N = 8), PE w/o MeHg (N = 12), PE with MeHg (N = 8), I N = 178. I Healthy N = 166, Rs = 0.51, P ≤ 0.0001; PE N = 12, Rs = 0.60, P = 0.0428; overall N = 178, Rs = 0.52, P ≤ 0.001; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-sided unpaired t test was used in (A–F). Two-sided unpaired t test was used in (G) for each time point. ANOVA followed by Tukey´s multiple comparisons test was used in (H). The Spearman correlation was used for the calculation in (I).
