Fig. 2: Mutual regulation of LAT1 and NRF2 affects oxidative stress and amino acid response of HTR-8/SVneo cells.
From: LAT1-NRF2 axis controls sFlt-1/PlGF imbalance and oxidative stress in preeclampsia
A Promoter activity of ABCC1 (MRP1) and SLC7A5 (LAT1) after NRF2 depletion. B NFE2L2 (NRF2) promoter activity and gene expression as well as nuclear NRF2 activity after LAT1 depletion. C ABCC1 (MRP1) gene expression after LAT1, or concomitant LAT1 and Keap1 depletion. D GSH/GSSG ratio of cells treated with medium, medium + 90 mg/L methionine or +3 mM N-acetylcysteine (NAC) after siRNA-mediated silencing of SLC7A5 (LAT1) and NFE2L2 (NRF2). E Total cellular GSH in LAT1 and NRF2 deficient cells. F GCLM mRNA levels upon LAT1 and NRF2 depletion. G Relative leucine and methionine uptake in LAT1 and NRF2 deficient cells. H ATF4 (ATF4) gene expression in cells cultured in control (ctrl) medium or methionine-free medium for 24 h. Data represent mean value ± SD. N = 3 (biological replicates). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Letters A–F denote homogeneous subgroups (P < 0.05) by post-hoc S-N-K test. Two-sided unpaired t test was used in (A, B, G, H). ANOVA followed by Dunnett´s multiple comparisons test was used in (C, E, F). ANOVA followed by S-N-K multiple comparisons test was used in (D).
