Fig. 3: Regulation of the sFlt-1/PlGF ratio during LAT1, and NRF2 deficiency.
From: LAT1-NRF2 axis controls sFlt-1/PlGF imbalance and oxidative stress in preeclampsia
A Expression levels of ATF4 (ATF4), Flt1 (sFlt-1), and PGF (PlGF), as well as Flt1/PGF mRNA ratio in HTR-8/SVneo cells. Tm: Tunicamycin B ATF4 expression in LAT1- and NRF2 deficient cells. C Levels of sFlt-1 and PlGF in medium of LAT1 and NRF2 deficient cells, as well as the sFlt-1/PlGF ratio. D sFlt-1/PlGF ratio of cells deficient for ATF4, LAT1, or both proteins. E sFlt-1/PlGF ratio of cells deficient for LAT1 and NRF2 or overexpressing the proteins. F GSH/GSSG ratio and sFlt-1/PlGF ratio in LAT1 deficient cells treated with 0.9 µM methyl mercury (MeHg) and 3 mM N-acetylcysteine (NAC). G Malondialdehyde (MDA) levels in placentas obtained from control or JPH203 (LAT1 specific inhibitor) treated pregnant mice. H Gene expression levels of ATF4 (ATF4) and NFE2L2 (NRF2) in placentas obtained from control or JPH203- treated pregnant mice. I Proteinuria determined in urine of control or JPH203-treated mice that was collected immediately after sacrificing. J Ratio of sFlt-1/PlGF-2 in plasma of control or JPH203-treated mice (8- to 16-week-old naturally cycling females) that was collected immediately after sacrificing. Data represent mean value ± SD. A–F: N = 3 (biological replicates), G–H N = 6, I ctrl: N = 4; JPH203: N = 6, J N = 6; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ANOVA followed by Dunnett´s or Tukey´s multiple comparisons test was used in (E, F). Two-sided unpaired t test was used in (G–J).
