Fig. 3: Sec31A inhibition induces a protective organismal response against ER stress.
From: Genome-wide CRISPR Screen Identifies Sec31A as a Key Regulator of Alpha Cell Survival
A UPRER stress response in C. elegans assessed by the transcriptional reporter hsp-4p::GFP and qualitative quantified by GFP intensity. B Bright field (BF) and GFP microscopy images of hsp-4p::GFP worms grown under COPII inhibition by KD of sec-23 and sec-31. Scale bar, 100 μm. C Quantification of the hsp-4 transcriptional response after RNAi against sec-31 (See Supplementary Data 4 for details). D Knockdown of sec-31 allowed a greater UPRER transcriptional response when subjected to ER stress by tunicamycin. Scale bar, 100 μm. E Quantification of biological replicates shown in (D). Data in (C, D) were analyzed using a two-sided Chi-square test to compare the frequency of fluorescent signal distribution between groups. No adjustments were made for multiple comparisons. F COPII inhibition by sec-31 KD allowed greater survival under ER stress conditions (see Supplementary Table 1 for survival details, replicates, and statistics). Numbers above bars denote sample size (biological replicates), **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.
