Fig. 5: Molecular analyses of human islet cells with SEC31A knock down.
From: Genome-wide CRISPR Screen Identifies Sec31A as a Key Regulator of Alpha Cell Survival
A Human alpha and beta cells sorted from cadaveric human islets were incubated in Miami media for seven days to generate pseudoislets. Created in BioRender. Shibue, K. (2025) https://BioRender.com/x9b8puh. B, C qPCR results showing SEC31A expression between control and SEC31AKD alpha (B) and beta (C) pseudoislets. Biological replicates, n = 4 for alpha cells and n = 3 for beta cells, each representing an independent biological replicate. Data are presented as mean values ± SEM. Two-tailed Student’s t test; *p < 0.05. D, E Representative images of control and SEC31AKD alpha pseudoislets (D) and beta pseudoislets (E). F, G Volcano plots comparing control and SEC31AKD alpha pseudoislets (F) and beta pseudoislets (G). Differential expression was analyzed using limma with linear modeling and moderated two-sided t tests, incorporating surrogate variables as covariates. FDR was controlled using the Benjamini-Hochberg method. H Heatmap of selected genes up-regulated in SEC31AKD alpha pseudoislets. Biological replicates, n = 4 for alpha cells and n = 2 for beta cells, each representing an independent biological replicate. I, J STRING analysis of the functional protein-protein interaction network of up-regulated genes in SEC31AKD alpha pseudoislets (n = 4, each representing an independent biological replicate)(I) and beta pseudoislets (n = 2, each representing an independent biological replicate)(J). K, L Enriched GO term analyses showing significantly up-regulated genes in SEC31A-KD alpha pseudoislets (n = 4, each representing an independent biological replicate) (K) and beta pseudoislets (n = 2, each representing an independent biological replicate)(L). Source data are provided as a Source Data file.
