Fig. 6: Sec31A cross talks with proteins in insulin/IGF1 signaling pathway.

A Co-immunoprecipitation of IRβ and Sec31A in alphaTC6 cells. Immunoblots of Sec31A in cell lysates that were immunoprecipitated with IRβ and Sec31A. B AlphaSe31AKD cells exhibited enhanced phosphorylation of insulin signaling pathways, independent of glucose concentration. Cells were treated with 100 nM insulin for 15 min following overnight starvation in DMEM containing either 5.5 mM or 25 mM glucose. The right panel displays the quantification of p-IR/IR and p-IRS1/IRS1 in the presence of 25 mM glucose. Data are presented as mean values ± SEM. (two-tailed Student’s t test). *p < 0.05, **p < 0.01. Three biological replicates cultured in independent wells were examined. C Repression of TG-induced cleaved caspase-3 elevation in alphaSec31A cells was not observed when TG was administered with OSI-906. Immunoblots of cleaved caspase-3 in control and alphaSec31A cells treated with/without TG and OSI-906. D Immunoblots of TCPTP in control and alphaSec31A cells after the treatment with TG. Quantification is shown in the lower panel. Data are presented as mean values ± SEM. (two-tailed Student’s t test). *p < 0.05. Six biological replicates cultured in independent wells were examined. Source data are provided as a Source Data file.