Fig. 3: miR-10a repolarizes macrophage phenotype by rescuing mitochondrial function and reshaping epigenetics.

a Schematic representation of the crosstalk between epigenetic changes and mitochondrial function mediated by miR-10a in macrophages. b Oxygen consumption rate (OCR) profile of M1 phenotype macrophages after miR-10a transfection. Data are presented as the mean ± SD (n = 3 biological replicates). c-f Basal respiration, ATP production, maximal respiration, and spare respiratory capacity of M1 phenotype macrophages following miR-10a transfection. Data are presented as the mean ± SD (n = 3 biological replicates). g, h Flow cytometry was used to analyze the ratio of respiratory and non-respiratory mitochondria to total mitochondria in M1 phenotype macrophages following miR-10a transfection. Data are shown as the mean ± SD (n = 3 biological replicates). i Immunofluorescence images of H3K9Ac (red) and F-action (green), with quantitative analysis of their average fluorescence intensity (j), in M1 phenotype macrophages after miR-10a transfection or treatment with the FAO inhibitor etomoxir, scale bar: 20 μm. Panel (j) data are presented as the mean ± SD (n = 20 fields from 5 independent samples). Contour plots and quantification of flow cytometry analysis showing the numbers of CD86-positive (k) and CD206-positive (l) cells following different treatments. Data are shown as the mean ± SD (n = 3 biological replicates). P values in (c-h and k-l) were determined by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. P values in (j) were determined by One-way ANOVA analysis followed by Dunnett’s T3 multiple comparisons test. Source data are provided as a Source Data file. Panel (a) was created in BioRender. Fang, F. (2025) https://BioRender.com/4u7xkq0.