Fig. 4: Prepared, characterization, and evaluation of the targeted capability of miR-10a@H-MNP. | Nature Communications

Fig. 4: Prepared, characterization, and evaluation of the targeted capability of miR-10a@H-MNP.

From: Reprogramming mitochondrial metabolism and epigenetics of macrophages via miR-10a liposomes for atherosclerosis therapy

Fig. 4

a Schematic diagram illustrating the design and preparation of miR-10a@H-MNP. b, c TEM and NTA were used to observe the morphology and size distribution of NP and H-MNP, respectively. These experiments were repeated three times and similar results were obtained. d Zeta potential measurements of NP and H-MNP. Data are shown as the mean ± SD (n = 3 biological replicates). e Co-localization of RBC membrane and cy5-miR-10a@H-MNP, scale bar: 20 μm. This experiment was repeated independently three times with similar results. f Coomassie brilliant blue staining showing the protein profile of the RBC membrane and H-MNP (RBC-M denotes RBC membrane). This experiment was repeated independently three times with similar results. g Western blot analysis detecting the expression of CD47 and Ter-119 in RBC membrane and H-MNP. This experiment was repeated independently three times with similar results. h miR-10a encapsulation efficiency of H-MNP. Data are shown as the mean ± SD (n = 3 biological replicates). i Stability of miR-10a@H-MNP against RNase A. j Release profile of cy5-miR-10a from H-MNP. Data are presented as the mean ± SD (n = 3 biological replicates). k Fluorescence images and mean fluorescence intensity of cy5-miR-10a@H-MNP or cy5-miR-10a@NP incubated with M1 phenotype macrophage with different times, scare bar: 20 μm. Data are shown as the mean ± SD (n = 5 biologically replicates). l Flow cytometry of cy5+ cells in M1 macrophages after incubation with cy5-miR-10a@H-MNP or cy5-miR-10a@NP. m IVIS imaging and quantification of radiation efficiency (n) in atherosclerotic mouse aortas post-intravenous injection of cy5-miR-10a@NP or cy5-miR-10a@H-MNP. Panel (n) data are shown as the mean ± SD (n = 3 independent samples). o Immunofluorescence staining image of aorta sections from atherosclerotic mice, 6 h post intravenous injection of cy5-miR-10a@NP (red) or cy5-miR-10a@H-MNP (red), with CD86 labeled macrophages (green) and DAPI labeled nuclei, scale bar: 20 μm. This experiment was repeated independently three times with similar results. Source data are provided as a Source Data file. P values in (d, h, k, and n) were determined by two-tailed unpaired Student t test. Panel (a) was created in BioRender. Fang, F. (2025) https://BioRender.com/u6enwo5.

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