Fig. 6: miR-10a@H-MNP reprograms macrophage phenotypes in vivo. | Nature Communications

Fig. 6: miR-10a@H-MNP reprograms macrophage phenotypes in vivo.

From: Reprogramming mitochondrial metabolism and epigenetics of macrophages via miR-10a liposomes for atherosclerosis therapy

Fig. 6

a CLSM images and fluorescence intensity quantification (b) of aortic root DHE staining (L denotes lumen). Data in panel (b) are shown as the mean ± SD (n = 20 sections from 5 independent samples). c IHC staining of aortic root macrophage markers CD68, M1 phenotype macrophage marker CD86, and M2 phenotype macrophage marker CD206 after various treatments, quantification of positive areas (red dotted frame) using image J software (d-f) (scale bar: 200 µm). Data in (d-f) are shown as the mean ± SD (n = 5 independent samples). g, h Flow cytometry analysis of the percentage of CD86- and CD206-positive macrophages in aortic tissue of ApoE−/− mice after treatment with miR-10a@H-MNP. Data are shown as the mean ± SD (n = 5 independent samples). The levels of IL-1β (i), TNF-α (j), and IL-6 (k) in the serum of ApoE−/− mice after different treatments. Data are shown as the mean ± SD (n = 5 independent samples). P values in (b and d-j) were determined by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. P values in (k) were determined by One-way ANOVA analysis followed by Dunnett’s T3 multiple comparisons test. Source data are provided as a Source Data file.

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