Fig. 2: Neuronal composition and spatial organization in the mouse and tree shrew sSC.
From: Co-Conservation of synaptic gene expression and circuitry in collicular neurons
A Visualization of neurons by species on the integrated UMAP (middle), with tree shrew neurons (left) and mouse neurons (right) shown in the same UMAP space. B Percentage of neurons in each neuronal subtype by each species (top), along with their expression of inhibitory (Gad2) and excitatory (vGluT2, bottom) markers. C Percentage of inhibitory and excitatory neurons in the integrated dataset. D Fluorescence in situ hybridization (FISH) of Gad1 and vGluT2 expression in the tree shrew SC. Left, laminar organization of SC is indicated according to the stereotaxic coordinates of the tree shrew brain (left). Right, a 1400 μm × 400 μm white rectangle is used to quantify expression and is further divided into 14 subregions along the SC depth. Scale bars: 500 μm. E Percentage of inhibitory and excitatory neurons labeled by FISH within the tree shrew and mouse sSC. n = 3 animals each. F Percentage of inhibitory and excitatory neurons along the SC depth based on RNA FISH in tree shrews (triangles) and mice (squares). G Neuronal density along the SC depth based on RNA FISH in tree shrews and mice.
