Fig. 3: TAF4A interacts with NF-Y and binds to the promoter of Kansl2.

a Heat maps of centered TAF4A CUT&RUN peaks. The blue-to-red gradient indicates high-to-low counts in the corresponding region. b Genome-wide distribution of TAF4A peaks. c Bar plot representing TAF4A peaks at different types of promoters. d Detected SP1 and NF-YA binding site under TAF4A CUT&RUN peaks. e Taf4a and Nf-Ya expression in freshly isolated MuSCs (fiMuSCs) and muscle based on reads per exon kilobase per million (RPKM) values (unpaired two-tailed t test: *p = 0.0103, ***p = 0.0005 n = 3). f Co-IP of TAF4A and NF-YA/B in wildtype MuSCs. Blots were probed with antibodies against TAF4A and NF-YA or NF-YB (n = 2). g Percentage of TAF4A CUT&RUN peaks overlapping either with NF-YA peaks or with SP1 binding motifs. h Venn diagram of overlapping TAF4A CUT&RUN (CnR) peaks, NF-YA CUT&RUN (CnR) peaks, and RNA-seq DEG in Taf4a^sKO MuSCs. i TAF4A, NF-YA and H3K4me3 distribution in the proximal promoter region of the Kansl2 gene in WT MuSCs. 8-20-weeks-old males and females were used. Data are presented as mean ± SEM of biological replicates. Source data are provided in the Source Data file.