Fig. 5: Paternal SARS-CoV-2 significantly alters the transcriptome of the hippocampus in F1 females and males.

A F1 female MA (Bland-Altman) plot of the Log₂(average expression + 1) for each gene vs. Log₂ fold-change relative to control values for each gene (red dots indicate genes which are FDR < 0.05), (B) Heatmap displaying the relative expression of the differentially expressed genes (FDR < 0.05) across all F1 female samples (n = 3 per paternal treatment group), (C) F1 female gene ontology pathways (y-axis) that were identified as significantly affected in the gene enrichment analysis of differentially expressed genes (gene symbols at the end of the bars indicating which gene/s are annotated to each term), (D) F1 male MA (Bland-Altman) plot of the Log₂(average expression + 1) for each gene vs. Log₂ fold-change relative to control values for each gene with a heatmap of differentially expressed genes below, (E) RNA-microinjected male MA (Bland-Altman) plot of the Log₂(average expression + 1) for each gene vs. Log₂ fold-change relative to control values for each gene with a heatmap of differentially expressed genes below, (F) RNA-microinjected female MA (Bland-Altman) plot of the Log₂(average expression + 1) for each gene vs. Log₂ fold-change relative to control values for each gene.