Fig. 3: Near-complete HLA matching restores long-term graft protection by allogeneic Tregs. | Nature Communications

Fig. 3: Near-complete HLA matching restores long-term graft protection by allogeneic Tregs.

From: HLA matching or CRISPR editing of HLA class I/II enables engraftment and effective function of allogeneic human regulatory T cell therapy in a humanized mouse transplantation model

Fig. 3

A Immunodeficient BALB/c Rag2−/− cγc−/− mice grafted with human skin received intraperitoneal human PBMCs (5 × 106) and either completely mismatched (1,2,1,2, n = 8), partially mismatched (0,1,1,2, n = 5), or partially matched (0,0,0,1, n = 5) Tregs (1 × 106) at HLA-A,-B,-C,-DR loci identified through screening over 150 distinct donors. Grafts were monitored for macroscopic signs of rejection over the subsequent 100 days. B Percentage of grafts surviving is plotted over time post-adoptive transfer of cells. The mice receiving partially mismatched Tregs developed signs of xenogeneic graft-versus-host-disease necessitating removal of these animals from the experiment at day 33. C Tabulated median survival time (defined as the time at which half of the grafts were rejected). Groups were analyzed using the two-tailed Mantel-Cox log-rank test with multiple testing correction using the Benjamini–Hochberg method (χ2(2) = 7.84, p = 0.0198); complete mismatch versus partial mismatch χ2(1) = 6.021, p = 0.0141; complete mismatch versus partial match χ2(1) = 4.376, p = 0.0364; partial mismatch versus partial match χ2(1) = 0.3254, p = 0.5684). Panel A created in BioRender. McCallion, O. (2025) https://BioRender.com/re81qq1. Source data are provided as a Source data file.

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