Fig. 1: Localization and transmission of Stammera capleta in tortoise beetles. | Nature Communications

Fig. 1: Localization and transmission of Stammera capleta in tortoise beetles.

From: Fidelity in co-diversified symbiosis

Fig. 1

A Adult tortoise beetle (Coleoptera: Cassidinae: Chelymorpha alternans). B Whole-mount fluorescence in situ hybridization (FISH) demonstrating S. capleta localization within each type of symbiotic organ. C Foregut symbiotic organs. D Ovary-associated glands. E Egg mass bearing caplets deposited at the anterior pole of each egg. F, G Whole-mount FISH of an egg and its caplet. H FISH cross-section of a caplet showing enclosed spheres, where S. capleta is embedded. Each image is from a single individual and is shown for illustrative purposes. Probes used: S. capleta (magenta: 16S rRNA) and the beetle host (blue: 18S rRNA). fg foregut, fso foregut symbiotic organs, mg midgut, hg hindgut, ov ovaries, oag ovary-associated glands, e embryo, c caplet, s S. capleta-bearing spheres, m caplet membrane. Scale bars are included for reference.

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