Fig. 2: Identification of centromeric regions in the HapOB reference genome.
From: A high-quality genome sequence of Rosa chinensis to elucidate ornamental traits
a, The cluster CL226 identified by RepeatExplorer. b, Agarose gel electrophoresis of tandem repeat fragments amplified from the genomic DNA of HapOB using OBC226 PCR primers (right lane) along with the lambda-PstI size ladder (left lane). Similar results were obtained in two independent experiments. c, FISH with carboxy tetramethylrhodamine (TAMRA)-labelled OBC226 oligo probes on R. chinensis metaphase chromosomes. Chromosome numbers are labelled from 1 to 7. Similar results were observed in at least 10 metaphase cells in two independent experiments. d, Circos representation of the distribution of OBC226 (purple), the pericentromeric region (blue), Ty3/Gypsy (orange) and Ty1/Copia repeat elements (green) along the seven pseudo-chromosomes and Chr0 (scale in Mb).
