Extended Data Fig. 4: Semi quantitative RT-PCR in plants ectopically expressing TTG1 WDR genes in the ttg1-1 mutant and in the triple mutant lwd1lwd2ttg1.
From: TTG1 proteins regulate circadian activity as well as epidermal cell fate and pigmentation
The figure shows for each sample 5 ul of the same amplification reaction after 20-25-35 PCR cycles. DNA ladder is 1kb hyperladder (Bioline). a, PCR of WT and ttg1 overexpressing lines (35S:TTG1, LWD1, LWD2, MpWDR1, MpWDR2) and reference gene (EUKARYOTIC TRANSLATION INITIATION FACTOR 4A1 (EIF4A1)). b, PCR of WT, triple mutant lwd1lwd2ttg1 and ttg1 overexpressing lines (35S:AmLWD, TTG1, LWD1, LWD2, MpWDR1, MpWDR2). Negative control samples indicated with “-“. Given the big differences observed, this experiment was performed only once, using multiple lines for most of the transgenic plants.
