Fig. 4: Phylogenetic affinities of genes horizontally transferred to A. angustus.

a, Phylogenetic tree of glyoxalase (PF13468). b, Phylogenetic tree of NAD-binding dehydrogenase (PF08635). c, Phylogenetic tree of glucuronyl hydrolase (PF07470). d, Phylogenetic tree of DNA methyltransferase (PF02870 and PF01035). The stars indicate that the Anthoceros sequence or bryophyte sequences formed a monophyletic clade with homologues of putative HGT donor, reflecting Anthoceros-specific or bryophyte-specific HGT events. Maximum-likelihood bootstrap support values ≥50% are shown above the branches. Red, hornworts and other bryophytes; cyan, green algae; grey, metazoan; orange, stramenopiles; blue, bacteria; yellow, fungi; purple, archaea. The homologues from the kingdom other than the one that HGT donors are involved in are used as the outgroup. The scale bars in the trees show the number of amino acid substitutions per site.