Extended Data Fig. 10: Synechocystis Rbp3 location and photosynthetic phenotype of the ∆rbp2/3 mutant.

a, Western blotting showing that Rbp3 is associated with the membrane fraction. See Source Data for full-length gel images with molecular weight markers. Data shown are representative of 2 biological replicates with similar results. b, growth curves for ∆rbp2/3 vs. the wild-type (GT-I) background in different conditions: 50 µmol photons m^-2s^-1 (low light), 80 µmol photons m^-2s^-1 (high light), or low light in the presence of 10 mM glucose. Error bars indicate standard deviations from 3 replicate cultures. c, absorption spectra of cell suspensions, normalised to turbidity at 750 nm. ∆rbp2/3 has lower pigment per OD₇₅₀. d, fluorescence emission spectra at 77K with chlorophyll excitation (435 nm) normalised to PSI emission at 725 nm. e, fluorescence emission spectra at 77K with phycocyanin excitation (600 nm) normalised to phycobilin emission at 660 nm. ∆rbp2/3 has lower PSI emission (725 nm) relative to PSII (peaks at 685 and 695 nm). All spectra are representative of similar results from 3 independent cultures.