Extended Data Fig. 1: Background fluorescence in fixed cells of Synechocystis and Synechococcus.
From: mRNA localization, reaction centre biogenesis and thylakoid membrane targeting in cyanobacteria
a, Confocal fluorescence images of photosynthetic pigments in Synechocystis cells showing thylakoid membrane organisation without and with the fixation and permeabilisation used for mRNA-FISH probing. b, statistics for cell diameter in fixed vs live cells (n = 50 cells: no adjustments for multiple comparisons). c, Confocal fluorescence images of photosynthetic pigments in Synechococcus cells showing thylakoid membrane organisation in live vs. fixed cells. d, e, statistics for cell width and length in fixed vs live cells (n = 50 cells: no adjustments for multiple comparisons). f, g, Confocal micrographs of Synechocystis (f) and Synechococcus (g) cells (fixed and permeabilised but not probed) showing the background signal (green) in the TAMRA detection channel in comparison to fluorescence from the photosynthetic pigments (TM, in red). Line profiles drawn from representative cells of both Synechocystis (f. i, ii) and Synechococcus (g, i) confirm that the background signal (green line) colocalises with the TM (black dashed line). TAMRA channel shown without background correction. Images are representative of at least 2 independent experiments. Error bars in the box plots indicate the range of values recorded, the centre line shows the median and the box spans the interquartile range. p values are from unpaired two-tailed Student’s t-tests. Scale bars: 2 µm.
