Extended Data Fig. 4: miPEP858a and miR858a regulate plant growth and development via auxin transport. | Nature Plants

Extended Data Fig. 4: miPEP858a and miR858a regulate plant growth and development via auxin transport.

From: Primary transcript of miR858 encodes regulatory peptide and controls flavonoid biosynthesis and development in Arabidopsis

Extended Data Fig. 4

a, Representative image of 10-day old WT and CRISPR/Cas9 edited miR858aCR, miR858bCR, miR858abCR, pre-miR858aCR, miPEP858aCR, OX miPEP858a and OX miR858a seedling grown on 1/2 strength Murashige and Skoog (MS) medium supplemented with ethanol (control) and 25 μM TIBA (scale bars, 1 cm). b, c, Root length of 10-day old WT and CRISPR/Cas9 edited miR858aCR, miR858bCR, miR858abCR, pre-miR858aCR, miPEP858aCR, OX miPEP858a and OX miR858a seedling lines grown on 1/2 strength Murashige and Skoog (MS) medium supplemented with ethanol (control) and 25 μM TIBA (n=30 independent seedlings, small open circles). d, e, Quantification of AUX1, PIN1, ABCB19 and YUC1 in 30-day old rosette of WT and CRISPR/Cas9 edited miR858aCR, miR858bCR, miR858abCR, pre-miR858aCR, miPEP858aCR, OX miPEP858a and OX miR858a. Statistical analysis was performed using two-tailed Student’s t-test. Data are plotted as means ±SD. Error bars represent standard deviation Asterisks indicate a significant difference between control and treatment, *P < 0.1, **P < 0.01, ***P < 0.001.

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