Extended Data Fig. 5: Allele-specific expression analysis of parent-of-origin genes in apical and basal cell lineages of early proembryos.
From: Equal parental contribution to the transcriptome is not equal control of embryogenesis
a–k, Sanger sequencing chromatographs of PCR products at selected SNP sites showing allelic expression pattern of parent-of-origin genes. cDNA of apical and basal cell lineages of early proembryos from the reciprocal crosses between Arabidopsis Col-0 and Ler were prepared for RT-PCR and Sanger sequencing. Genomic DNA (gDNA) of Arabidopsis Col-0, Ler and F1 hybrids from the reciprocal crosses between Col-0 and Ler were used as the controls. The polymorphic nucleotide was underlined in each chromatogram. BEGs, biparentally expressed genes.
