Extended Data Fig. 6: In vitro and in planta competition assays of V. dahliae with VdAve1-insensitive bacterial isolates.
From: Microbiome manipulation by a soil-borne fungal plant pathogen using effector proteins
a, VdAve1 does not contribute to V. dahliae colonization in vitro in the presence of Ralstonia sp., P. corrugata or A. tumefaciens. Letters represent non-significant biomass differences (one-way ANOVA and Tukey’s post-hoc test; p<0.05; N=12). b, Tomato seed treatment with Ralstonia sp. does not reduce Verticillium wilt symptoms. Phenotypes of tomato plants 14 days post inoculation with wild-type V. dahliae or the VdAve1 deletion mutant. Tomato seeds were surface-sterilized and allowed to germinate in vitro in the presence or the absence of Ralstonia sp. prior to infection. c, Canopy area of mock-treated and Ralstonia-treated tomato plants infected by wild-type V. dahliae or the VdAve1 deletion mutant. ((one-way ANOVA and Tukey’s post-hoc test; p<0.05; N=20). d, V. dahliae biomass in tomato stems determined with real-time PCR. Letters represent significant biomass differences (one-way ANOVA and Tukey’s post-hoc test; p<0.05; Sample sizes of experimental conditions from left to right are N=20, N=18, N=20 and N=18, respectively. Whiskers of the boxplots as shown in a,c,d display the upper and lower quartile, the boxes display the interquartile range and the thick line displays the median.
