Extended Data Fig. 8: Function and expression analysis of CNGC18 in mlo5/9.
From: Integration of ovular signals and exocytosis of a Ca2+ channel by MLOs in pollen tube guidance
a, Transcription of CNGC18 in mlo5/9 is not affected. 1, 2 and 3 are pollen tubes from three different plants of the annotated genotype. b, Overexpression of CNGC18-GFP can partially rescue the impaired pollen tube growth of mlo5/9. Bar, 1 mm. n=3 biologically independent experiments for a–b were repeated with similar results. c, Pollen tube length of different transgenic lines of mlo5/9 expressing CNGC18-GFP. Data are mean±s.e.m. n=17, 12, 14, 16, 11 and 9 pistils for each genotype. Two-tailed Students’t-test, p***=0.000299153, p***=0.000558313, p***=0.000566278, p***= 0.000576008 for 1# to 4#, respectively. d, e, NMT analysis showing the Ca2+ flux of two transgenic lines (1# and 2#) compared with the same mlo5/9 pollen tube. n=9 pollen tubes were measured for each genotype. n=3 independent experiments for d, e were repeated with similar results.
