Extended Data Fig. 9: Triple mutants generated by CRISPR technology.
From: Integration of ovular signals and exocytosis of a Ca2+ channel by MLOs in pollen tube guidance
a, Mutations of MLO15 in two different alleles (mlo15-1 and mlo15-2). b, Semi-in vitro pollen tube length of mlo5/9 and mlo5/9/15-1 mutants. Data are mean±s.e.m. Two-tailed Students’ t-test, p***=4.39×10-5, p***=0.000569674 for 1# and 2#. n=23, 19, 18, 14, 15 pistils for each genotype. c, Representative images of in vivo pollen tube length of mlo5/9 and mlo5/9/15-1 mutants at 3, 5 and 8 hours after pollination (HAP). Bar, 200 μm. d, Quantification of c. Data are mean±s.e.m. Two-tailed Students’t-test, p*=0.0378, p**=0.005820983, p**=0.001425173 for comparison between mlo5/9 and mlo5/9 /15-1 at 3HAP, 5HAP, 8HAP, respectively. n.s. No statistical significance. p=0.27263357 for WT and mlo5/9 at 8HAP. n=19, 17, 18, 16, 10, 18, 20, 19 and 19 pistils for each sample.
