Extended Data Fig. 1: MLOs expression, mutant characterization and phenotyping.
From: Integration of ovular signals and exocytosis of a Ca2+ channel by MLOs in pollen tube guidance
a, Real-time PCR analysis showing the expression of MLO members in root and pollen. Data are mean±s.e.m. of 3 replicates. b, Phylogenetic tree of MLO family. c, T-DNA insertion sites in mlo5 and mlo9 (left panel). Arrow, site of primers used for the RT-RCR analysis in d, Right pane, protein topology and the corresponding mutation sites of the mutants. d, RT-PCR analysis of the mutants. e, Seed set of WT, mlo5-1, mlo5-3, mlo9, and double mutants. Bar, 2 mm. f, Aniline-blue stained pollen tubes of mlo5-1 and mlo9 at 8 hours after pollination on WT stigma. Bar, 200 μm. n=3 (for d) and 6 (for e and f) biological independent experiments were performed with similar results. g, Quantification of pollen germination ratio of the WT and mlo5-1 mlo9. Data are mean±s.e.m, n=1000 pollen for each genotype. Two-tailed Students’ t-test. p=0.7465. n.s. No statistical significance.
