Extended Data Fig. 2: Expression and subcellular localization of MLO5 and MLO9.
From: Integration of ovular signals and exocytosis of a Ca2+ channel by MLOs in pollen tube guidance
a, b, Confocal imaging of pollen grains from MLO5:MLO5-GFP transgenic plants. a, GFP channel; b, Merged GFP and bright channels. c, Confocal imaging of pollen tubes from MLO5:MLO5-GFP transgenic plants. d–f, The mutated MLO5 fails to target to the plasma membrane (d and e) and cannot rescue the shortened mlo5/9 pollen tubes (f). e, Fluorescence intensity of d. Bar in a–d, 5 μm; f, 1 mm. n=6 independent biological experiments were performed for a–f with similar results.
