Extended Data Fig. 2: Molecular cloning of PINNA1 gene and cosegregation analysis.
From: A molecular framework underlying the compound leaf pattern of Medicago truncatula
a, Simplified schema of the Tnt1 insertion site-based mapping strategy. Red and blue short-lines represent the recovered Tnt1 FSTs of two different mutant lines. b, Images of representative WT, pinna1-1, -2, -3, -4 and -5 leaves. This experiment was repeated independently three times with similar results. Scale bar, 1 cm. c, The detailed positions (red numbers indicate position from the first ATG codon) of Tnt1 insertion sites in pinna1 alleles. Yellow shaded letters indicate the exon sequences. The consensus GT/AG splice sequences are boxed. d, PINNA1 gene structure, the Tnt1 insertion site of pinna1-1 and the primers (arrowheads) used for genotyping. e, Cosegregation analysis of a F2 population derived from a cross of pinna1-1 x WT (R108). 41 out of 172 F2 individuals showing mutant phenotype were homozygous for Tnt1 insertion. The primer pair Fw100-RvTAG100 was used for detecting intact PINNA1 genomic fragments (upper); the primer pair Fw394-TntR2 was used for verifying the existence of Tnt1 insertion within the PINNA1 genomic region (lower). This experiment was repeated independently three times with similar results.
