Extended Data Fig. 8: Heritable editing frequency increases as the infected plant matures.
From: Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
a, Green and bleached plant phenotypes of progeny from parental plants infected with a TRV vector expressing sgNbPDS-tRNAIleu, sgNbPDS-tRNAMet, or sgNbPDS-tRNAGly. About 50–60 progeny seeds from a pod from the top of the parent plants (top panels), a pod from middle of the parent plants (middle panels) and a pod from base of the parent plants (bottom panels) were planted on soil in individual pots. Pictures were taken 10–14 days post-seeding. Similar phenotypes were observed in two independent experiments. b, Ratio of bleached progeny to total number of progeny from individual seed pods. Parental plants were infected with a TRV vector expressing sgNbPDS (Unmodified sgRNA, n = 3 independent biological replicates),sgNbPDS-Truncated FT (TRV2-NbPDS-AtFT, n = 3), sgNbPDS-tRNAIleu (n = 2), sgNbPDS-tRNAMet (n = 2), or sgNbPDS-tRNAGly (n = 2). Seeds were collected from each seed pod individually with Seed Pod 1 being the first emerged seed pod and ascending vertically. Thirty to sixty progeny were phenotyped per pod. Three infected parent replicates were collected per treatment. Each shape corresponds to one infected parent replicate. Each line corresponds to the mean percentage of bleached progeny from each modified or unmodified sgRNA treatment.
