Extended Data Fig. 9: Growth conditions affect heritable editing frequency.
From: Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
Bleached progeny (%) indicates the ratio of bleached progeny to green progeny. Parental plants were infected with a TRV vector expressing sgNbPDS (TRV2-sgNbPDS) or, sgNbPDS-Truncated FT (TRV2-sgNbPDS-AtFT). Progeny were collected from the entire infected parent plant and 150–300 seedlings were phenotyped. TRV2-sgNbPDS infected plants were grown at two different conditions: 22 °C with 24 h light (n = 2 independent biological replicates, \(\bar x\) = 0.004 ± 0.005) or 26 °C day/22 °C night with a 12 hour day/night cycle (n = 3, \(\bar x\) = 0.034 ± 0.039). All TRV2-sgNbPDS-AtFT were grown at 26 °C day/22 °C night with a 12 hour day/night cycle (n = 3, \(\bar x\) = 0.421 ± 0.125). Each dot represents one plant replicate. Bars indicate the mean editing frequency; error bars represent ± standard deviation.
