Extended Data Fig. 1: Addition of Flowering Locus T sequences to sgRNAs does not inhibit editing activity.

There are no significant differences in editing efficiency when the sgRNA is either unmodified (n = 5 independent biological replicates, \(\bar x\) = 0.73 ± 0.27) or modified with FT (n = 3, \(\bar x\) = 0.61 ± 0.19, p-value= 0.477), mFT (n = 5, \(\bar x\) = 0.87 ± 0.05, p-value= 0.328), or truncated FT (n = 3, \(\bar x\) = 0.88 ± 0.03, p-value= 0.286). The modified or unmodified sgRNAs were expressed from TRV vectors, and tissue was collected from the infiltrated site in N. benthamiana leaves. Collecting tissue from the infiltrated site allows for examination of sgRNA function alone. DNA was extracted from the infiltrated site two weeks after infiltration. The targeted locus was PCR amplified and sequenced using Next Generation Sequencing (NGS). The Y axis denotes the fraction, displayed as a percentage, of reads with indels at the targeted locus divided by the total number of reads. Each dot represents one plant replicate. Bars indicate the mean editing frequency; error bars represent ± standard deviation. Asterisks indicate statistically significant differences compared with the unmodified sgRNA by the two-sided Student’s t-test, NS > 0.05, *<0.05, **<0.01.