Extended Data Fig. 3: Inheritance of gene edits at the N. benthamiana AG locus.
From: Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
a, Genotypes of progeny from parents with observed +T/-7bp mutations. One seedling (M1) produced from a parent plant (M0) infected with TRV vectors expressing a sgRNA with Truncated FT had +T/-7bp mutations in AG. This plant was grown to maturity and seeds were collected from the first seed pod. Nineteen progeny of this plant were genotyped, and an approximate Mendelian inheritance was observed. b, Genotypes of progeny from parents with observed +T/ + T mutations. One seedling (M1) from a parent plant (M0) infected with TRV vectors expressing a sgRNA with truncated FT had +T/ + T mutations in AG alleles. This plant was grown to maturity and seeds were collected from the first seed pod. Nineteen progeny of this plant were genotyped, and the expected Mendelian inheritance was observed. Genotypes were determined through DNA extraction of each progeny, amplification of the AG locus, Sanger sequencing, and detection of indels by ICE (Synthego). Due to noise in ICE analyses, the following parameters were used to call genotype: 0%-10%=WT, 35%-65%= heterozygous (WT/indel), 85%-100%=bi-allelic mutation.
