Extended Data Fig. 4: Phenotypes of plants infected with TRV vectors expressing sgRNAs that target N. benthamiana PDS.
From: Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
Pictures of plants were taken between four and eight weeks after infection. The pictures display some of the typical phenotypes observed from either a, a non-infected plant or plants infected with a TRV vector expressing b, a non-augmented sgRNA, c, an FT-augmented sgRNA, d, a mFT-augmented sgRNA, e, a truncated FT-augmented sgRNA, f, a glycine tRNA-augmented sgRNA, g, a methionine tRNA-augmented sgRNA, h, an isoleucine tRNA-augmented sgRNA. Similar phenotypes were observed in three independent experiments, and each experiment consisted of 2–3 independent plants.
