Extended Data Fig. 5: TRV2 abundance reflects heritable editing efficiency.
From: Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
a, Relative TRV2 RNA levels were measured by quantitative real-time PCR relative to the control, PP2A. b, Green and bleached plant phenotypes of progeny from parental plants infected with a TRV2 vector expressing sgNbPDS, sgNbPDS-tRNAIleu, sgNbPDS-tRNAMet, or sgNbPDS-tRNAGly. Frequencies of heritable editing correlate with viral abundance in the parent generation. About 50–60 progeny seeds from a pod from the top of the parent plants were planted on soil in individual pots. Pictures were taken 10–14 days post-seeding. This experiment was repeated twice with similar results.
