Extended Data Fig. 2: Nup93a preys identified by conventional IP-MS.
From: Global profiling of plant nuclear membrane proteome in Arabidopsis
a, Nuclear localization of Nup50c-YFP in leaf epidermal cells during transient expression in N. benthamiana. Free mCherry was coexpressed. The nucleus (N) is marked with arrowhead. The localization pattern has been repeated in two independent experiments with similar results. Bar = 10 μm. b, Nup93a-BioID2-HA preys identified by conventional immunoprecipitation (IP) using HA antibody followed by LFQMS. YFP-BioID2-HA and HA-BioID2-NEAP1 plants were used as controls for ratiometric analysis. The integrated peptide intensity data of all samples were normalized and subjected to ratiometric analysis and plotting. Nup93 preys are defined by cutoffs fold-change > 2 and p-value < 0.05 compared to both controls (linear model F-test, n = 2 biologically independent samples). c, Statistics for Nup93a preys identified by the conventional IP-MS approach in b.
