Extended Data Fig. 5: Physical and genetic interactions of PNET1/6 with nucleoporins.
From: Global profiling of plant nuclear membrane proteome in Arabidopsis
a, Fluorescence images of BiFC between PNET1 and Nup35, Nup88, Nup93a, Nup58, and Nup155. The indicated BiFC constructs and free mCherry were coexpressed in N. benthamiana. The localization patterns have been repeated in two independent experiments with similar results. Bars = 10 μm. b, Interactions of PNET6 with nucleoporins using BiFC assay performed in N. benthamiana. The relative BiFC intensity was obtained by normalizing BiFC fluorescence using averaged expression levels of the corresponding Nup-YFP measured in separate experiments. Results are presented as boxplots with first quartile, median, and third quartile (n = 12). Ac, accessory nucleoporin; IRC, inner ring complex; FG, nucleoporins that contain Phe-Gly repeats; ORC, outer ring complex; Linker, linker nucleoporins. Membrane, membrane nucleoporins. c, Quantification of silique length of six-week-old plants. Data are presented as mean ± standard deviation (n = 15). Similar results have been obtained by an independent batch of samples.
