Extended Data Fig. 6: Genotyping of M0 plants regenerated from plant tissues infected with SYNV vector targeting the gS3–2 site.
From: Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
a, Detection of target mutations and viral vector in independent M0 plants by PCR-RE and RT-PCR assays. The size of undigested DNA fragments (red arrowhead) and digestion products are labeled, M, 500-bp ladder DNA marker. b, M0 plant genotypes determined by Sanger sequencing. M0 plants with tetra-allelic mutations are labeled in blue letters. Deleted nucleotides are denoted by dash symbols, and inserted nucleotides or substitutions are shown in boldface and italicized letters, respectively. Mutation type: WT, wild-type sequence with no mutation; d#, i#, and s# denote number of bases deleted, inserted, or substituted at target site, respectively.
