Extended Data Fig. 7: Segregation of phenotypes and genotypes in M1 and M2 progeny derived from five representative M0 lines bi-allelic for PDS-A and PDS-B.
From: Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
For each M0 line, the genotypes of one albino and four normal M1 progeny were determined by Sanger sequencing (Supplementary Figure 5). The seeds produced by the normal M1 plants after self-pollination were germinated on MS media plates and the M2 progeny phenotypes counted. P values were calculated by using a chi-squared test of goodness-of-fit to segregation at a 3:1 or 15:1 ratio. 0.1< P <0.5, in good agreement with theoretical segregation ratios; P >0.5, in very good agreement with theoretical segregation ratios. d# and i# denote # of bp deleted and inserted from the target site, respectively, and the in-frame deletion types (d3, d6, and d9) are highlighted in green letters. Ho, homozygote; Bi, bi-allele; n/a, not applicable.
