Extended Data Fig. 9: Detection of potential off-target effects.
From: Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
DNA fragments encompassing the gPDS-1 on-target (PDS-A) and 13 potential off-targets (OT1 to OT13; see Supplementary Table 5) were each amplified from the albino M0–11 plant by PCR with specific primers and then subjected to digestion with T7EI and HinfI (if applicable). Lanes 1, 2, and 3 show samples from a mock-infected plant without or with enzyme digestion, and from the M0–11 plant with enzyme digestion, respectively. M, ladder DNA marker.
